BloodReady™ – 1mL - Atelerix

BloodReady™ is designed for the encapsulation of whole blood in a simple, scalable vial format.

BloodReady 1ml

£245.00

Title

International Delivery Available

Title

Includes gelBASE beads, non-crosslinked gel and dissolution buffer for the in situ encapsulation, storage and release of whole blood in a vial.

WHY CHOOSE BLOODREADY?

Preserve your whole blood sample for up to 3 days.

Extend the stability of fresh whole blood from hours to days, allowing flexible transportation, processing, and analysis.

Preserve all leukocyte populations and cellular markers, without the need for fixation or cryopreservation.

Scalable technology to preserve between 500 µl - 10 mL whole blood.

QUICK AND EASY TO USE

Encapsulate your sample by simply adding a gel concentrate to your whole blood sample and transferring to a vial containing gelation beads. After a few minutes, the whole blood and Gel mixture will gel in situ and is ready for storage or shipment. When ready to use, a gentle Dissolution Buffer is added to dissolve the gel, the beads are filtered out, and the whole blood sample ready to use.

ENCAPSULATION PROCESS

PRODUCT CODE

COMPONENTS

UNITS

UNIT VOLUME

BLOOD VOLUME TO ADD

BLR-01S-03

BLR-01S-06

BLR-01S-12

BLR-01S-24

BLR-01S-50

GelBase Beads

Gel A (5x)

Dissolution Buffer

MACS SmartStrainers

GelBase Beads

Gel A (5x)

Dissolution Buffer

MACS SmartStrainers

GelBase Beads

Gel A (5x)

Dissolution Buffer

MACS SmartStrainers

GelBase Beads

Gel A (5x)

Dissolution Buffer

MACS SmartStrainers

GelBase Beads

Gel A (5x)

Dissolution Buffer

MACS SmartStrainers

3 tubes

3

6 tube

6

12 tube

12

24 tube

24

50 tube

50

1 mL

0.25 mL

2 mL

1 mL

0.25 mL

2 mL

1 mL

0.25 mL

2 mL

1 mL

0.25 mL

2 mL

1 mL

0.25 mL

2 mL

-

1 mL

-

1 mL

-

1 mL

-

1 mL

-

1 mL

-

APPLICATIONS

i) Allow granular immunophenotyping for major and rare subpopulations.

Comprehensive immunophenotyping shows that BloodReady preserves the cell viability of major and rare immune cell populations in whole blood including: T Cells (CD3+), Helper T cells (CD4+), Cytotoxic T cells (CD8+), Regulatory T cells (CD25+ CD127-/dim), Gamma delta T cells (TCRγδ+), and Natural Killer T cells (CD56+). NK Cells (CD56+ CD16+), CD57+ NK cells, Early NK cells (CD56+ CD16-), and Mature NK cells (CD56+ CD16+). B Cells (CD19+), Transitional B cells (CD27- IgD+), Non-Switched B Cells (CD27+ IgD+), and Switched B cells (CD27+ IgD-). Innate Lymphoid Cells (ILC) (CD127+), ILCs CD2+ CD4+, and ILCs CD2+ CD4-. Monocytes (CD4+ HLA-DR-), Classical Monocytes (CD14+ CD16-), Intermediate Monocytes (CD14+ CD16+), and Non-Classical Monocytes (CD14- CD16+). Granulocytes (SSChi, CD15+ CD11b+ cells).

ii) Improve epitope preservation compared to fixation

BloodReady^TMpreserves the expression of >90% epitopes screened on lymphocytes, >80% on monocytes and >70% on granulocytes. Comparison with major competitor fixative solution Cyto-Chex shows that BloodReady^TMpreferably preserves several epitopes including CD62L, CD317, CD82, CD367, and integrin b7, while also not limiting downstream application due to fixation.

iii) Isolate live functional PBMCs

Storage of whole blood in BloodReady improves PBMC yield, post-rest recovery, and response to IL-2 stimulation when returned to culture. BloodReady^TM is a suitable storage solution for subsequent PBMC isolation and application.